{
  "schema_version": "1.0",
  "project": {
    "code": "RO-024",
    "name": "FND–miRNA 量子生物传感",
    "owner": "Xiaoyu Wang",
    "status": "active",
    "updated": "2026-07-28",
    "objective": "建立兼顾荧光增强、量子可读性、微流控富集和复杂样本稳定性的检测方案，并形成可复核的论文证据链。"
  },
  "research_design": {
    "question": "能否在增强 FND 荧光信号的同时保持 ODMR 可读性，并利用微流控富集提高低丰度 miRNA 的有效检测事件数？",
    "hypothesis": "适度金属耦合与受控间距能够提高光子收集效率；将 PL 与微波响应联合判读，可降低仅靠强度检测产生的假阳性。",
    "variables": {
      "independent": [
        "界面结构",
        "微柱几何",
        "靶标浓度",
        "样本基质"
      ],
      "dependent": [
        "PL",
        "ODMR contrast",
        "Q-SBR",
        "事件数",
        "CV"
      ]
    }
  },
  "evidence": [
    {
      "id": "L-01",
      "topic": "量子读出",
      "claim": "FND 的 ODMR 对比度可作为微波可寻址身份信号。",
      "project_role": "支撑核心读出机制",
      "status": "verified"
    },
    {
      "id": "L-02",
      "topic": "金属界面",
      "claim": "局域电磁场可能增强荧光，但需同时检查自旋可读性。",
      "project_role": "定义界面优化边界",
      "status": "verified"
    },
    {
      "id": "L-03",
      "topic": "微流控",
      "claim": "循环流动可增加低丰度靶标与捕获区的接触机会。",
      "project_role": "支撑富集结构设计",
      "status": "verified"
    }
  ],
  "tasks": [
    {
      "id": "EXP-01",
      "type": "experiment",
      "title": "完成三种界面结构的 PL 对比",
      "owner": "Xiaoyu",
      "due": "2026-07-26",
      "acceptance": "3 批 × 3 片，输出原始值与 CV",
      "status": "done"
    },
    {
      "id": "EXP-02",
      "type": "experiment",
      "title": "测试三种界面的 ODMR contrast",
      "owner": "Xiaoyu",
      "due": "2026-07-28",
      "acceptance": "相同微波功率与曝光参数",
      "status": "done"
    },
    {
      "id": "SIM-01",
      "type": "simulation",
      "title": "完成 CPW 四 ROI 磁场均匀性扫描",
      "owner": "Simulation",
      "due": "2026-07-25",
      "acceptance": "四区 |B| CV 小于 5%",
      "status": "done"
    },
    {
      "id": "SIM-02",
      "type": "simulation",
      "title": "比较 5 × 5 与 7 × 7 微柱流场",
      "owner": "Simulation",
      "due": "2026-07-31",
      "acceptance": "输出混合指数与 t90",
      "status": "todo"
    },
    {
      "id": "EXP-04",
      "type": "experiment",
      "title": "补做血清基质第 3 批重复",
      "owner": "Xiaoyu",
      "due": "2026-07-30",
      "acceptance": "与 PBS 使用同一归一化流程",
      "status": "todo"
    },
    {
      "id": "ANA-01",
      "type": "analysis",
      "title": "冻结量子门控事件判据",
      "owner": "Data",
      "due": "2026-07-27",
      "acceptance": "保存阈值、误判率和版本说明",
      "status": "done"
    }
  ],
  "assets": [
    {
      "id": "DATA-01",
      "path": "raw/pl_interface_batch01.csv",
      "version": "v1",
      "source_task": "EXP-01",
      "status": "locked"
    },
    {
      "id": "DATA-03",
      "path": "processed/event_gate_results.csv",
      "version": "v3",
      "source_task": "ANA-01",
      "status": "frozen"
    },
    {
      "id": "FIG-03",
      "path": "figures/figure03_pl_odmr.png",
      "version": "v2",
      "source_task": "FIG-03",
      "status": "review"
    },
    {
      "id": "MANU-01",
      "path": "manuscript/main_text.docx",
      "version": "v7",
      "source_task": "WRT-01",
      "status": "active"
    }
  ],
  "risks": [
    {
      "id": "R-01",
      "level": "high",
      "title": "复杂基质重复不足",
      "action": "完成 EXP-04 后再冻结复杂基质结论。"
    },
    {
      "id": "R-02",
      "level": "medium",
      "title": "Figure 3 版本未冻结",
      "action": "确认 PL 与 ODMR 的归一化方式。"
    },
    {
      "id": "R-03",
      "level": "medium",
      "title": "期刊范围待确认",
      "action": "根据实验完整度完成最终选刊。"
    }
  ]
}